Figure 4.

Cyclic signal transducer and activator of transcription 3 (STAT3) decoy (CS3D) exhibits a longer half-life in human serum than linear STAT3 decoy (S3D). (A,C) Biotinylated S3D and CS3D were incubated in human serum samples #1 or #2 and aliquots were removed at varying time intervals, electrophoresed on urea/polyacrylamide gels, and stained with SYBR Gold. (B,D) Densitometric analyses were performed on biotinylated S3D and CS3D pulled down from serum. Stability at each time point was expressed as a percent relative to its respective “undigested” control. Undigested control represents decoy suspended in 1X phosphate-buffered saline (PBS) and lacking any serum. Each time point of the S3D and CS3D serum stability assays was performed in triplicate for each sample of human serum.