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. 2018 Jun 6;19(6):1691. doi: 10.3390/ijms19061691

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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BBR decreased NEFA-induced TG accumulation in bovine hepatocytes. Oil red O staining (×40): (A) The normal bovine hepatocytes; (B) The bovine hepatocytes were pretreated with 1.2 mmol/L NEFA for 9 h; (C) After NEFA treatment, the hepatocytes were treated with 10 μmol/L BBR for another 24 h; (D) After NEFA treatment, the hepatocytes were treated with 20 μmol/L BBR for another 24 h; (E) BBR treatment significantly reduced the NEFA-induced increase in TG content. Quantified data are mean ± SD; ** p < 0.01 versus control group; ^## p < 0.01 versus NEFA group.