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. 2018 May 23;19(6):1555. doi: 10.3390/ijms19061555

Figure 3.

Figure 3

Alterations in mitochondrial respiratory chain complexes in RHM. Protein samples were extracted and subjected to Western blotting. Changes in mitochondrial complexes were detected using the Total OXPHOS Rodent WB Antibody Cocktail. (A): changes in complex V alpha subunit (CV-ATP5A-55 kDa), (B): complex III core protein 2 (CIII-UQCRC2-48 kDa), (C): complex IV subunit I (CIV-MTCO1-40 kDa), (D): complex II subunit 30 (CII-SDHB-30 kDa), (E): complex I subunit NDUF88-20 kDa (CI-NDUFB8). Immunodetection of Tom20 was used as a loading control. The upper parts of panels represent immunostaining with an OXPHOS antibody cocktail and Tom20. The lower parts show the quantification of immunostaining using computer-assisted densitometry. Bar graphs represent the levels of appropriate complexes with respect to the Tom20 level in absolute units. The data are presented as the means ± SD. * p < 0.05 significant difference in the protein level in comparison with the corresponding control.