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. 2018 Jun 12;19(6):1736. doi: 10.3390/ijms19061736

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Protective effects of berberine (BBR) against H₂O₂-induced cytotoxicity in D407 cells. (A) The structure of BBR; (B) D407 cells were treated with BBR (0.3 to 30 µM) or 0.1% dimethyl sulfoxide (DMSO) (vehicle control) for 24 h and cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Cells were pre-treated with BBR at indicated concentration or 0.1% DMSO (vehicle control) for 2 h and then incubated with or without 100 µM H₂O₂ for further 24 h. Cell viability and the release of lactate dehydrogenase (LDH) were measured by MTT assay (C) and LDH assay (D), respectively. * indicates p < 0.05, ** indicates < 0.01, *** indicates p < 0.001 versus the control group; ^# indicates p < 0.05, ^## indicates p < 0.01 versus the H₂O₂-treated group were considered significantly different.

Protective effects of berberine (BBR) against H₂O₂-induced cytotoxicity in D407 cells. (A) The structure of BBR; (B) D407 cells were treated with BBR (0.3 to 30 µM) or 0.1% dimethyl sulfoxide (DMSO) (vehicle control) for 24 h and cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Cells were pre-treated with BBR at indicated concentration or 0.1% DMSO (vehicle control) for 2 h and then incubated with or without 100 µM H₂O₂ for further 24 h. Cell viability and the release of lactate dehydrogenase (LDH) were measured by MTT assay (C) and LDH assay (D), respectively. * indicates p < 0.05, ** indicates < 0.01, *** indicates p < 0.001 versus the control group; ^# indicates p < 0.05, ^## indicates p < 0.01 versus the H₂O₂-treated group were considered significantly different.