Figure 3.

BBR attenuated H₂O₂-induced loss of mitochondrial membrane potential (Δψm) and activation of caspase 3/7 in D407 cells. After pre-treatment with 3 μM BBR or 0.1% DMSO (vehicle control) for 2 h, D407 cells were incubated with or without 100 μM H₂O₂ for another 24 h. (A) Δψm was determined by JC-1 assay; (B) Quantification of caspase 3/7 activity was determined by caspase 3/7 activity assay. ** indicate p < 0.01, *** indicate p < 0.001 versus the control group; ^## indicate p < 0.01, ^### indicate p < 0.001 versus the H₂O₂-treated group were considered significantly different.