Figure 6.

AMPK specific inhibitor Compound C blocked the protective effect of BBR in D407 cells. (A) D407 cells were pre-treated with 5 µM Compound C for 30 min and 3 µM BBR for 2 h and then incubated with or without H₂O₂ for a further 24 h. Cell viability was measured by MTT assay. * indicates p < 0.05. ** indicates p < 0.01, *** indicates p < 0.001 was considered significantly different; (B) D407 cells were pre-treated with 5 µM Compound C for 30 min and 3 µM BBR for 80 min and then incubated with or without H₂O₂ for a further 2 h. The expression of phosphorylated AMPK, total AMPK, and β-actin were detected by Western blotting with specific antibodies; (C) Quantification of representative protein band from Western blotting. * indicates p < 0.05versus the H₂O₂ group was considered significantly different. ^# indicates p < 0.05 versus the BBR + H₂O₂-treated group was considered significantly different.