Figure 8.

BBR protected primary cultured human RPE cells against H₂O₂ induced injury via the AMPK pathway. (A) Primary cultured hRPE cells were pre-treated with different concentrations of BBR for 2 h, then incubated with or without H₂O₂ for a further 24 h. Cell viability was measured by MTT assay. ** indicates p < 0.01 versus the control group was considered significantly different, ^# indicates p < 0.05, ^## indicates p < 0.01 versus H₂O₂ group was considered significantly different; (B) Primary cultured hRPE cells were pre-treated with 5 µM Compound C for 30 min and 3 µM BBR for 2 h and then incubated with or without H₂O₂ for further 24 h, and cell viability was measured using the MTT assay. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 was considered significantly different.