Figure 3.
Deletion of MSMEG_1285 in Msmeg mc2155 impacts the cell-surface exposure of HBHA_Mtb. AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for (A) the wild-type strain of Msmeg, (B) Msmeg mc2155 expressing hbhA_Mtb, (C) the ΔMSMEG_1285 mutant and (D) the ΔMSMEG_1285 mutant expressing hbhA_Mtb. All the strains were cultured in 7H9 supplemented with OADC and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.