TABLE 2.
TRUE HYPOXIA-REGULATED GENES IN B16(F10) MURINE MELANOMA CELL LINE
| Affymetrix Probeset ID | Gene Symbol | Gene Name | Fold-Change (Hypoxia vs. Control) |
|---|---|---|---|
| Top 20 upregulated probesets | |||
| 95706_at | Lgals3 | lectin, galactose binding, soluble 3 | 19.41 |
| 100596_at | Selenbpl* | selenium binding protein 1 | 13.65 |
| 104184_at | Nppb | mouse BNP gene for brain natriuretic peptide, complete cds. | 13.08 |
| 98478_at | Ccng2 | cyclin G2 | 7.985 |
| 93294_at | Ctgf | connective tissue growth factor | 7.468 |
| 104337_f_at | Pkp2 | plakophilin 2 | 6.967 |
| 97834_g_at | Pfkp | phosphofructokinase, platelet | 6.943 |
| 93836_at | Bnip3 | BCL2/adenovirus E1B 19 kDa-interacting protein 1, NIP3 | 6.936 |
| 103520_at | Vegfa | vascular endothelial growth factor A | 6.138 |
| 102798_at | Adm | adrenomedullin | 6.023 |
| 160495_at | Ahr | aryl-hydrocarbon receptor | 5.765 |
| 94004_at | Cnn2 | calponin 2 | 5.528 |
| 104338_r_at | Pkp2 | plakophilin 2 | 5.442 |
| 93738_at | Slc2al | solute carrier family 2 (facilitated glucose transporter), member 1 | 5.364 |
| 10155l_s_at | Tes | testis derived transcript | 5.151 |
| 98496_at | Gys3 | glycogen synthase 3, brain | 5.134 |
| 96803_at | Gbel | glucan (1,4-alpha-), branching enzyme 1 | 5.016 |
| 104701_at | Bhlhb2 | basic helix–loop–helix domain containing, class B2 | 4.742 |
| 100509_at | Rnfl9* | ring finger protein (C3HC4 type) 19 | 4.64 |
| 97870_s_at | Eroll | EROl-like (S. cerevisiae) | 4.416 |
| Top 20 downregulated probesets | |||
| 104586_at | Sfrsl | splicing factor, arginine/serine-rich 1 (ASF/SF2) | 0.282 |
| 103821_at | Cdc6 | cell division cycle 6 homolog (S. cerevisiae) | 0.3 |
| 95063_at | Cdca7* | cell division cycle associated 7 | 0.34 |
| 97445_at | Ppid* | peptidylprolyl isomerase D (cyclophilin D) | 0.34 |
| 103412_at | 3732412D22Rik | RIKEN cDNA 3732412D22 gene | 0.34 |
| 103922_f_at | Cyb5rl | cytochrome b5 reductase 1 | 0.38 |
| 94340_at | 1110004L07Rik | exportin, tRNA (nuclear export receptor for tRNAs) | 0.39 |
| 97538_at | Gus | beta-glucuronidase | 0.4 |
| 93320_at | Cptla | carnitine palmitoyltransferase 1a, liver | 0.41 |
| 103665_at | Elovl6* | ELOVL family member 6, elongation of long chain fatty acids (yeast) | 0.42 |
| 95135_at | Midlipl | Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) | 0.42 |
| 92582_at | Slcla5* | solute carrier family 1 (neutral amino acid transporter), member 5 | 0.42 |
| 93237_s_at | Tyms-ps* | thymidylate synthase | 0.425 |
| 160878_at | Bopl* | block of proliferation 1 | 0.43 |
| 95462_at | Bzw2 | basic leucine zipper and W2 domains 2 | 0.44 |
| 99620_at | Sfpq* | splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) | 0.44 |
| 103619_a | Cyb5b | cytochrome b5 type B | 0.45 |
| 96340_at | Tmem50b | transmembrane protein 50B | 0.45 |
| 100026_at | Bcatl* | branched chain aminotransferase 1, cytosolic | 0.45 |
| 160723_at | 1500001M20Rik | RIKEN cDNA 1500001M20 gene | 0.46 |
Genes selected by comparing the “true hypoxia” group to control samples (FWER < 0.05, Westfall-Young algorithm). Only the genes with the highest fold-change are shown (full list available in the Web Appendix, Table A).
*
Genes not previously associated with hypoxia.