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QUANTITATIVE REAL-TIME PCR VALIDATION OF MICROARRAY RESULTS
| Gene | Mean Hypoxia | Mean Control | p-Value | Fold-Change |
|---|---|---|---|---|
| Adm | 54.98 | 0.27 | p < 0.005 | 206.4 |
| Lgals3 | 57.87 | 0.52 | p < 0.005 | 112.2 |
| Nppb | 138.26 | 1.48 | p < 0.005 | 93.2 |
| Vcl (selected by limma method) | 16.09 | 1.15 | p < 0.005 | 14.0 |
Expression of the four genes chosen from the examined microarray datasets was normalized to that of the reference gene (P4hb). The statistical significance of results was assessed using Kolmogorov-Smirnov test.
