SUPPLEMENTARY TABLE 2.

VALIDATION OF MICROARRAY DATA BY Q-PCR

Gene Symbol	Microarray		Q-PCR −ΔΔCt
	Difference (TPA − Control)	Log2 Ratio (TPA/Control)	1 h	3 h
Group I (difference >200)
FOS	595.75	3.00	3.03*	4.36*
TNF	1399.75	2.80	1.79*	4.32*
IL8	1536.05	2.70	3.22*	6.61*
JUN	343.35	1.30	1.10*	3.04*
NFKBIA	1320.85	1.20	0.79*	1.13*
HSPCA (Hsp90A)	939.80	0.50	−0.05	−0.03
Group II (difference 50–200)
DDX3X	56.80	0.80	0.05*	1.22*
SKIL (SnoN)	55.35	0.70	0.03	1.73*
CCL2	61.00	0.60	0.35*	1.23*
SLC7A1	60.65	0.60	−0.16*	0.85*
NFIL3	59.65	0.60	0.64*	2.56*
BCLAF1 (BTF)	55.90	0.50	−0.12*	0.80*
Group III (difference 30–50)
PDE4B	31.70	0.90	0.08	0.74*
C14orf92	44.70	0.70	−0.29	0.13
LIMD1	33.45	0.70	−0.33*	−0.91*
TAF1	33.15	0.70	−0.43*	1.09*
KIAA0286	38.95	0.50	−0.16*	0.43*
JMJD3	34.75	0.50	0.32	2.00*

Eighteen genes showing upregulation in primary microarray analysis of CHX + TPA-treated and CHX + vehicle control were selected for quantitative real-time PCR analysis to validate the primary selection criteria. The Q-PCR is displayed as the differential cycle threshold (indicated as −ΔΔCt) for each gene for triplicate independent experiments.

^*Significant change in expression (p < 0.05).