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Primer specificity for Aurora family members. To detect the gene expression pattern of each Aurora family member, specific primers for Aurora-A, -B, and -C were designed for quantitative RT-PCR using Aurora family members that were constructed on the pCMV2 vector (A) and mRNA templates from the UB37 bladder cancer cell line as an example (B). The amplified products were then subjected to agarose gel followed by ethidium bromide staining.
