TABLE 5.

HORMONE-INDUCED VEGF EXPRESSION IN WT1- AND DDS-LNCaP CELLS RELATIVE TO V-LNCaP CELLS^*

Cells	5 nM R1881†		0 nM R1881†
	24 h	48 h	24 h	48 h
WT1-LNCaP	1.9-fold decrease	4.9-fold decrease	1.2-fold decrease	3.6-fold decrease
DDS-LNCaP	2.7-fold increase	5.9-fold increase	0.56-fold increase	1.2-fold decrease

^*Using quantitative real-time PCR, VEGF transcript levels were normalized to cyclophilin levels as described in Table 4. Normalized VEGF mRNA levels in transfected cells (WT1-LNCaP or DDS-LNCaP) were calibrated against VEGF levels in V-LNCaP cells using comparative Ct analysis (22) as described in the text.

^†Cells were synchronized by serum starvation then treated with 0 or 5 nM R1881 for 24 or 48 h prior to RNA isolation.