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. 2018 Jul 5;13(4-5):271–282. doi: 10.3727/000000006780666966

Figure 5.

Figure 5

Effect of MLL knockdown on Keratin 8 mRNA levels in MCF-7, SK-BR-3, T-47D, and MCF-10A cells. Cells were transfected with MLL siRNA (+) or with control siRNA (−) and harvested 72 h after transfection. (A) Treatment with MLL siRNA results in a decrease of MLL mRNA levels compared to controls. Results are expressed as the percent ratio relative to Keratin 8 mRNA level in control cells. All data are presented as the means ± SD of three independent experiments. (B) Treatment with MLL siRNA results in a decrease of MLLc protein levels compared to controls. Whole cell lysate was subjected to Western blotting using anti-MLLc antibody. β-Actin served as the loading control. (C) Analyses of the effect of MLL knockdown on Keratin 8 mRNA levels. Results are expressed as the ratio relative to the Keratin 8 mRNA level in control MCF-10A cells. All data are presented as the means ± SD of three independent experiments. *p < 0.0001 versus control by Student’s t-test.