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Effect of MLL knockdown on Histone H3 K4 trimethylation within the Keratin 8 gene. MCF-7 (A), SK-BR-3 (B), T-47D (C), and MCF-10A (D) cells were transfected with MLL siRNA or unrelated control siRNA and used for ChIP assays 72 h after transfection. The amount of precipitated DNA was quantified relative to input as described in Materials and Methods. All data are presented as the means ± SD of two independent experiments. The initiation site of TATA box is designated at +1.
