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Purification of cellulase from crude sample of Paenibacillus sp.
| Purification steps | Volume (ml) | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) | Purification fold |
|---|---|---|---|---|---|---|
| Crude extract | 1500 | 300 | 81,788 | 273 | 100 | 1 |
| 80% ammonium sulfate saturation | 60 | 160 | 75,121 | 469 | 91.8 | 1.7 |
| DEAE-cellulose column chromatography | 55 | 40 | 64,525 | 1613 | 78.9 | 5.9 |
| CM-cellulose column chromatography | 25 | 17 | 29,210 | 1718 | 35.7 | 9.7 |
