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. 2018 Jul 4;9:181. doi: 10.1186/s13287-018-0918-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Outline of the CR method for establishing equine keratinocytes cultures and potential uses of generated cells. Equine tissues were obtained from different horses; tissues were digested enzymatically followed by initiation of cell growth and propagation using irradiated mouse fibroblast and ROCK inhibitor. Cells were cryopreserved in liquid nitrogen. The generated equine keratinocytes can be used in numerous applications, including - but not limited to - induced reprogramming stem cells generation (iPSC), transdifferentiation into somatic cells, drug screening, and regenerative medicine