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. 2018 Jul 4;9:181. doi: 10.1186/s13287-018-0918-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Equine keratinocyte growth was tested in various culture conditions with or without 10 uM Y-27632. (a) Growth curve was generated to represent growth rate by plotting population doubling over days in time (graph shows the average of replicates). The arrow indicates cells that continued to divide indefinitely. Phase contrast images of primary equine keratinocytes (EK-1547) cultured in co-culture with irradiated fibroblasts, CNT, F, and KSFM with (b) or without Y-27632 (c). All images were taken on day 2 and day 7 following initial culture without passage (day 5 for feeders+Y condition due to 95% confluency) (×10 magnification. Size bars = 400 μm). Top right images show enlarged magnification (×40 magnification, size bars = 100 μm)