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CRMP2 and CRMP4 are expressed in the retinal ganglion cell layer when retinal axons are exiting the eye and crossing the midline. Whole mount in situ hybridization was performed in zebrafish embryos using RNA probes for CRMP2. (a, b) CRMP2 and CRMP4 transcripts are detected in the retina (white arrows) at 36 hpf. (c, d) CRMP2 and CRMP4 transcripts are detected in the retina of 48 hpf embryos (white arrows). Scale bar: 50 μm.
