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. 2018 Jul 5;16:72. doi: 10.1186/s12915-018-0538-z

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© Borowsky et al. 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Hoechst 33342 dye treatment typically reveals three subpopulations of hybrid male sperm in flow cytometry. a Sperm from a hybrid male form one population in 0.4× Hanks balanced saline. b Sperm from the same male treated with Hoechst 33342 dye (40 μM) form three subpopulations indicated by arrows