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. 2018 Mar 30;93(7):1901–1915. doi: 10.1002/jctb.5599

Figure 8.

JCTB-5599-FIG-0008-b

Effect of sodium acetate pH 4 buffer concentration on the (a) desorption of bound rabbit Ig and total protein from washed type III MEP‐linked magnetic adsorbents in a single elution cycle and (b) corresponding SDS‐PAGE analysis. The support concentration during binding was 24.6 mg mL‐1 and was reduced to 19.5 mg mL‐1 for washing and elution. The amounts of Ig and total protein released are expressed as percentages of the total bound prior to elution. Key: desorbed Ig (white up‐triangles), desorbed protein (black up‐triangles); Ig purity (grey up‐triangles).