Table 2.
Summary of data for magnetic rack based recovery of Ig from clarified rabbit antiserum (10% v/v) using type III MEP‐linked magnetic adsorbents. Two sets of conditions (A and B) were investigated
| Condition A. Support concentration of 30 mg mL‐1 during binding and washing and 60 mg mL‐1 during elution | |||||||
|---|---|---|---|---|---|---|---|
| Recovery step | Ig (mg) | Protein (mg) | Purity (%) | Ig yield (%) | Protein yield (%) | PF (‐)* | YF (‐)** |
| Antiserum | 3.95 | 14.19 | 27.8 | 100 | 100 | 1.0 | 1.0 |
| Unbound | 0.27 | 6.93 | 3.9 | 6.8 | 48.8 | ||
| Bound | 3.68 | 7.26 | 50.6 | 93.2 | 51.2 | 1.82 | 1.70 |
| Wash | 0.10 | 1.00 | 10.0 | 2.5 | 7.0 | ||
| Elution 1 | 1.68 | 2.27 | 73.7 | 42.5 | 16.0 | 2.65 | |
| Elution 2 | 0.94 | 1.24 | 75.8 | 23.9 | 8.8 | 2.73 | |
| Combined elutions | 2.62 | 3.52 | 74.4 | 66.4 | 24.8 | 2.68 | 1.78 |
| Mass balance (%) | 75.7 | 80.7 | |||||
| Elution efficiency (%) | 73.2 | 56.2 | ‐ | ||||
| Condition B. Support concentration of 24.6 mg mL‐1 during binding and 19.5 mg mL‐1 during washing and elution | |||||||
|---|---|---|---|---|---|---|---|
| Recovery step | Ig (mg) | Protein (mg) | Purity (%) | Ig yield (%) | Protein yield (%) | PF (‐)* | YF (‐)** |
| Antiserum | 3.95 | 14.19 | 27.8 | 100 | 100 | 1.0 | 1.0 |
| Unbound | 0.39 | 7.38 | 5.3 | 9.9 | 52.0 | ||
| Bound | 3.56 | 6.81 | 52.2 | 90.1 | 48.0 | 1.88 | 1.69 |
| Washes | 0.13 | 0.98 | 13.6 | 3.4 | 6.9 | ||
| Elution 1 | 2.57 | 3.44 | 74.9 | 65.2 | 24.2 | 2.68 | |
| Elution 2 | 0.58 | 0.77 | 75.0 | 14.7 | 5.4 | 2.70 | |
| Combined elutions | 3.15 | 4.21 | 74.9 | 79.9 | 29.6 | 2.68 | 2.14 |
| Mass balance (%) | 93.3 | 88.6 | |||||
| Elution efficiency (%) | 92.1 | 72.2 | |||||
*
PF = overall purification factor and
**
YF = yield factor.