Table 3.

Summary of data for HGMF based recovery of Ig from unclarified rabbit antiserum (10% v/v) using type III MEP‐linked magnetic adsorbents. The concentration of support particles employed during binding was 31.7 mg mL^‐1 during binding and 23.5 mg mL^‐1 during washing and elution

Recovery step	Ig (mg)	Protein (mg)	Purity (%)	Ig yield (%)	Protein yield (%)	PF (‐)*	YF (‐)**
Antiserum	28.7	107.6	26.67	100	100	1.0	1.0
Flow through (unbound)	1.35	43.52	3.10	4.7	40.4
Bound	27.35	64.08	42.7	95.3	59.6	1.60	1.52
Wash	1.35	8.29	16.3	4.7	7.7
Elution 1	15.40	19.01	81.0	53.6	17.7	3.04
Elution 2	5.38	6.70	80.3	18.7	6.2	3.01
Combined elutions	20.78	25.71	80.8	72.4	23.9	3.03	2.19
Mass balance (%)	81.8	72.0
Elution efficiency (%)	79.9	46.1

^*PF = overall purification factor and

^** YF = yield factor.