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. Author manuscript; available in PMC: 2018 Jul 5.
Published in final edited form as: Cell Rep. 2018 May 1;23(5):1553–1564. doi: 10.1016/j.celrep.2018.03.133

Figure 1. IDH1R132H Promotes Growth of Immortal Astrocytes, Consumes NADPH, and Increases 2-HG Levels.

Figure 1

(A) Cell proliferation was determined by MTT assay of astrocytes derived from N::TVA;Cdkn2alox/lox; Atrxlox/lox;Ptenlox/lox mice and infected with viruses containing Cre, IDH1R132H, IDH1, and/or PDGFA as indicated.

(B) Anchorage-independent growth of N::TVA; Cdkn2alox/lox;Atrxlox/lox;Ptenlox/lox astrocytes infected with viruses containing the genes indicated was analyzed by soft agar colony formation assay.

(C) NADPH production was measured from N::TVA;Cdkn2alox/lox;Atrxlox/lox;Ptenlox/lox astrocytes infected with viruses containing the genes indicated.

(D) 2-HG production from N::TVA; Cdkn2alox/lox; Atrxlox/lox;Ptenlox/lox astrocytes infected with viruses containing the genes indicated was detected by LC/MS.

(E) Anchorage-independent growth of N::TVA; Cdkn2alox/lox;Atrxlox/lox;Ptenlox/lox astrocytes infected with viruses containing the genes indicated and/or treated with 2-HG (checked bars) was analyzed by soft agar colony formation assay.

Data are expressed as the mean ± SEM with three replicates. Statistical significance is denoted: *p < 0.05, **p < 0.01, ***p < 0.001. See also Figure S1.