(A) Cell proliferation was determined by MTT assay of astrocytes derived from N::TVA;Cdkn2alox/lox; Atrxlox/lox;Ptenlox/lox mice and infected with viruses containing Cre, IDH1R132H, IDH1, and/or PDGFA as indicated.
(B) Anchorage-independent growth of N::TVA; Cdkn2alox/lox;Atrxlox/lox;Ptenlox/lox astrocytes infected with viruses containing the genes indicated was analyzed by soft agar colony formation assay.
(C) NADPH production was measured from N::TVA;Cdkn2alox/lox;Atrxlox/lox;Ptenlox/lox astrocytes infected with viruses containing the genes indicated.
(D) 2-HG production from N::TVA; Cdkn2alox/lox; Atrxlox/lox;Ptenlox/lox astrocytes infected with viruses containing the genes indicated was detected by LC/MS.
(E) Anchorage-independent growth of N::TVA; Cdkn2alox/lox;Atrxlox/lox;Ptenlox/lox astrocytes infected with viruses containing the genes indicated and/or treated with 2-HG (checked bars) was analyzed by soft agar colony formation assay.
Data are expressed as the mean ± SEM with three replicates. Statistical significance is denoted: *p < 0.05, **p < 0.01, ***p < 0.001. See also Figure S1.