Figure 2.

Experimentally determined and predicted binding affinities for HLA‐A2‐restricted HPV16 E6/E7‐derived peptides. In silico predictions were conducted with 11 web‐based algorithms. For clarity, only results from NetMHC 4.0 are displayed. Experimental binding affinities were determined with in vitro competition‐based binding assays. The order of peptides is from strongest (top) to weakest (bottom) binding affinity as determined in the in vitro assays. Strong binders: experimental IC₅₀ below 5 μm; intermediate binders: experimental IC₅₀ 5–15 μm (marked with light gray); weak binders: experimental IC₅₀ > 15–100 μm (marked with dark gray). Results are plotted as mean ± SD from at least three experimental replicates.