Figure 2.

Sclerostin upregulates in the articular cartilage post‐injury. Sost immunostaining was conducted on contralateral WT (A) and SOST^TG (E and I) joints at 1 day after injury. Injured WT joints had elevated levels of Sost (B and C), while injured SOST^TG joints had elevated expression of both mouse (F and G) and human Sclerostin (J and K) 1 day after injury. No differences were observed in Sclerostin expression in the osteocytes of injured animals (D, H, and L). Real‐time quantitative PCR (qPCR) analysis of whole‐joint RNA of WTs at 1, 2, 3, and 4 days after injury (M). All images are at ×20 magnification. *p < 0.05, **p < 0.01.