Table 1.
Theileria annulata and bovine proteins identified with confidence in the myc‐BirA*‐CLASP11256–1538 experiment
| Number of unique peptides | |||||||
|---|---|---|---|---|---|---|---|
| ID | Description | Cellular location (function) | %Coverage | #PSM | Protein Mass (Da) | TaC12 | TaC12_myc‐BirA*‐CLASP11256–1538 |
| Q4UCH5 | T. annulata putative uncharacterized protein (TA03615) | Secreted, host cell cytoplasm/schizont membrane | 19.51 | 17 | 90499.741 | 10 | |
| L0 L373 | T. annulata major schizont surface protein p104 (TA08425) | Secreted, schizont plasma membrane | 10.26 | 20 | 101040.604 | 5 | |
| E1BGU2 | CLASP1 | Cytoplasm (MT plus‐end tracking protein, promotes stabilisation of dynamic MTs) | 5.62 | 49 | 162254.491 | 8 | |
| E1BQ15 | CLASP2 | Cytoplasm (MT plus‐end tracking protein, promotes stabilisation of dynamic MTs) | 1.88 | 23 | 168182.483 | 1 | |
| E3W9A2 | CAP‐Gly domain‐containing linker protein 1 (CLIP‐170) | Cytoplasm (MT plus‐end tracking protein) | 3.92 | 2 | 160624.438 | 2 | |
| A6QR54 | Janus kinase and microtubule‐interacting protein 1 (Jakmip1) | cytoplasm (binds to MTs and Jak family members) | 3.51 | 2 | 73271.288 | 2 | |
| Q28046 | Adseverin | Cytoplasm (Ca2+‐dependent actin filament severing protein) | 22.38 | 107 | 80577.919 | 8 | |
| H7BWW0 | Coronin 1B | Cytoplasm (actin filament nucleation, inhibition of actin filament depolymerization) | 18.24 | 19 | 54252.78 | 5 | |
| P79136‐2 | F‐actin‐capping protein subunit β (isoform β‐2) (CapZB) | cytoplasm (blocking exchange of subunits at barbed ends of actin filaments) | 20.22 | 10 | 30628.743 | 3 | |
| Q5E9F5 | Transgelin‐2 | Cytoplasm (actin cytoskeleton) | 20.1 | 7 | 22426.497 | 2 | |
| F1MDH3 | Talin‐1 | Cytoplasm (connects actin filaments with integral membrane proteins) | 3.53 | 4 | 270815.461 | 3 | |
| P19803 | Rho GDP‐dissociation inhibitor 1 (Arhgdia) | Cytoplasm (regulation of small GTPases) | 45.59 | 8 | 23421.4 | 4 | |
| F1MM14 | CD2‐associated protein (CD2AP, CMS) | Cytoplasm (actin binding, adaptor protein) | 46 | 85 | 71203.755 | 23 | |
| F1MVN9 | SH3 domain‐containing kinase‐binding protein 1 (CIN85) | Cytoplasm (adaptor protein) | 5.09 | 8 | 68540.403 | 2 | |
| E1B7Z1 | Arf‐GAP with SH3 domain, ANK repeat and PH domain‐containing protein 1 (ASAP1) | cytoplasm (GAP activity, phosphorylated by Src kinase, adaptor protein) | 5.05 | 7 | 123079.556 | 2 | |
| G3N016 | Crk‐like protein (CRKL) (predicted) | Cytoplasm (signal transduction) | 12.99 | 4 | 33386.587 | 3 | |
| F1MFD5 | Ran GTPase‐activation protein 1 (RanGAP) (predicted) | Cytoplasm, nuclear pore complex (stably interacts with RanBP2) | 12.31 | 8 | 62942.186 | 3 | |
| F1MXS1 | E3 SUMO‐protein ligase RanBP2 | Interphase: nuclear pore complex (mRNA export) Mitosis: centromere (important for chromosome segregation). | 38.58 | 208 | 336780.654 | 1 | 72 |
| F1MH31 | nuclear pore complex protein Nup214 isoform (predicted) | Nucleus (nuclear pore complex), cytoplasmic in mitosis | 4.87 | 7 | 211599.748 | 5 | |
| E1BIW0 | E3 ubiquitin‐protein ligase UBR1 | Cytoplasm (protein ubiquitination) | 2.81 | 6 | 199466.17 | 3 | |
Note. Two T. annulata proteins (TA03615 and TA08415) were identified by mass spectrometry that were absent from the control. Seventeen bovine proteins were identified with one or more peptide hit in the myc‐BirA*‐CLASP11256–1538 sample that were absent from the control. One bovine protein (FIMXS1) was identified by 72 peptides, with one peptide identified in the control. Uniprot and GeneDB were used to assign accession numbers (ID), description, and cellular localisation. The %coverage value indicates the percentage of the protein that is covered by the peptides identified by the mass spectrometry analysis. PSM is peptide spectral match, and #PSM reflects the total number of identified peptides for one particular protein. The #peptides column lists the number of different peptides found for each protein. MT = microtubule.