Table 2.
The costs of running some of the more prominent single‐cell technologies. Note that prices are estimates and may vary as a result of different suppliers, exchange rates and prices scalable on quantity purchased. None of these costs include sequencing, see Table 1
| scRNA‐Seq | Paired heavy‐light chain | ||||
|---|---|---|---|---|---|
| Drop‐Seq | 10x genomicsc | Smart‐seq | Overlap‐extension | 10x Genomicsc | |
| Equipment cost | US$50 000‐65 000b | US$75 000 | N/Aa | US$55 000b | US$75 000 |
| Per run cost | US$500‐700 | US$1288 | US$1000 | US$400‐500 | US$1288 |
| Cells per run | ~10 000 | 100‐100 000 | 96‐384 | 100 000‐150 000 | 100‐100 000 |
| Estimated time to process a run (h) | 24‐48 | 24‐48 | 48‐72 | 24‐48 | 24‐48 |
| Capture efficiency | 5‐10% | 65% | 100% | >90% | 65% |
Although Smart‐seq does not require any specialized equipment it does require the ability to sort cells into 96 or 384 well plates.
This cost is based on an ‘off the shelf’ model although methods exist for self‐assembly. For Drop‐Seq and Ig pairing by overlap extension we have used Dolomite Bio as our reference. In this case as well, buying the equipment for one method will reduce the equipment purchase price for the other as parts are interchangeable.
The 10X system uses the same machine for both methods. Note that the system will also perform both scSeq and paired heavy light chain from the same sample for US$65 more and TCR on top of that at an additional US$65.