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. Author manuscript; available in PMC: 2018 Jul 5.
Published in final edited form as: Methods Mol Biol. 2015;1298:331–354. doi: 10.1007/978-1-4939-2569-8_28

Fig. 2.

Fig. 2

Quantitative measurements of GTPase-effector protein binding. (a) Flow cytometry-based measurement of total Rab7 binding to RILP by detecting fluorescent BODIPY-GTP shows binding is saturable, quantitative, and specific. Rab7 binding to GSH beads coated with GST alone or without any protein coating was minimal. (b) Specific binding of Rab7-RILP with unwanted nonspecific background binding subtracted