Micro-computed tomography image analysis of the rat proximal tibia. (A) The bone volume fraction (BV/TV) determined within the trabecular bone compartment of the proximal tibia for the naïve, contusion, and transection groups (mean ± standard error of the mean). Statistical analyses (one-way analysis of variance, post-hoc Tukey) determined no statistically significant differences between any of the groups. (B) Representative three-dimensional (3D), computer-generated 16-bit volumes depicting BV/TV region of interest (ROI) evaluated for the naïve, contusion, and transection groups; the dark gray mask represents the original cropped volume (∼1.8-mm below the epiphyseal line), the white mask represents the 3-mm high ROI evaluated, and the gray mask inside the cortical boundary represents the generated 3D trabecular structure.