FIG. 3.

Host to donor connectivity. (A) After injection of 1 μL, 10,000 molecular-weight BDA into the ipsilateral host gray matter at C1/2, a few BDA-positive fibers (red) can be seen near the center of the transplant (green). High magnification of a BDA fiber is seen in (B). (C) Cross-section through the epicenter of the transplant that has been immunohistochemically stained for the presence of serotonin and counterstained with cresyl violet. (D) A high-magnification image shows host serotonergic axons innervating the transplant near the graft-host border. Scale is 500 um for low power images (A and C) and 20 um for high-power images (B and D). Dashed lines outline the cross-section of the spinal cord (A) and delineate the graft-host border (C). 5HT, serotonin; BDA, biotin dextran amine; GFP, green fluorescent protein. Color image is available online at www.liebertpub.com/neu