FIG. 7.

Immunohistochemical characterization of transplanted neurons. Low-power images of cross-sections through the GFP⁺ transplant epicenter can be seen in (A), (F), and (H), which have been immunohistochemically stained for NeuN and glutaminase (A, B, C, D, and E), TH (F and G) and GAD65/67 (H and I) in red. High-magnification insets reveal examples of glutaminase-positive, TH-positive, and GAD65/67-positive donor neurons (E, G, and I, respectively). A high degree of GAD-65/67-positive nerve terminals can be seen throughout the entire transplant, closely resembling what is seen throughout host grey matter. There are also pockets of high-density GAD-67/65 labeling in the transplant as is normally seen in the host dorsal horns, suggesting that some degree of long-term, cytoarchitecture fate is maintained. Scales are as indicated. GAD65/67, glutamic acid decarboxylase 65/67; GFP, green fluorescent protein; NeuN, neuronal nuclear protein; TH, tyrosine hydroxylase. Color image is available online at www.liebertpub.com/neu