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. Author manuscript; available in PMC: 2018 Jul 5.
Published in final edited form as: Cancer Res. 2018 Jan 16;78(6):1497–1510. doi: 10.1158/0008-5472.CAN-17-2090

Figure 1.

Figure 1

Matrix deprivation promotes Akt inactivation concomitant with AMPK activation. Representative immunoblots of the following cell lysates were probed for specified proteins. A, Freshly isolated HMECs cultured in attached condition (Att) or as floating mammospheres (MS) in ultralow-attachment plates for 7 days. B–C, MDA-MB-231 (B) and MCF7 (C) cells cultured in attached condition (Att) or as anchorage-independent spheroids (AI-spheroids) in methylcellulose for 7 days. D–E, MDA-MB-231 cells cultured in attached condition (Att) or subjected to suspension (Sus) for 8 hours on poly-HEMA-coated plates. Graphs represent densitometric quantification of immunoblots; error bars, mean ± SEM; n = 3.