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. Author manuscript; available in PMC: 2018 Jul 5.
Published in final edited form as: Cancer Res. 2018 Jan 16;78(6):1497–1510. doi: 10.1158/0008-5472.CAN-17-2090

Figure 4.

Figure 4

Downregulation of PHLPP2 promotes anoikis and impairs autophagy and metastasis. A–D, MDA-MB-231 cells stably expressing nontargeting shRNA (NT) or shPHLPP2 (seq #5) were subjected to 48 hours of suspension in the presence of DMSO or LY294002 (LY) and harvested for caspase-3 activity assay (A), subjected to soft-agar colony formation (B), and AO assay (C and D); n = 3. E and F, MDA-MB-231 cells stably expressing control GFP vector or GFP-tagged HA-Akt-T308D S473D (GFP Akt DD) were subjected to suspension (Sus) for 48 hours and harvested for AO assay (E) and immunoblotting (F); n = 3. G and H, Graphs represent number of lung (left) and liver (right) nodules following tail-vein injection of cells described below; each dot represents number of nodules in a single mouse: G, Nontargeting shRNA (NT) or shPHLPP2 (seq #5). H, Control GFP vector or GFP-tagged HA-Akt-T308D S473D (GFP Akt DD). Error bars, mean ± SEM in all experiments.