Figure 2. Verification of down-regulated proteins in the attenuated strain M. bovis-150.
RT-qPCR analysis revealed down-regulation of several genes (Mbov_0070, 0312, 0306, 0512, 0593, 0730, 0797) during attenuation of M. bovis HB0801. Data are presented as mean ± SD. Representative image was selected from three independent experiments performed in triplicate. β-actin was used as the internal control.