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. 2017 Nov 2;9(47):28322–28336. doi: 10.18632/oncotarget.22265

Figure 3.

Figure 3

Cloning of M. bovis Mbov_0730 and site directed mutagenesis with overlap extension PCR (A), Six fragments of Mbov_0730 was cloned by PCR. (B) the whole gene was cloned using six fragments of Mbov_0730 as a template. “M” indicated gene marker (2000 bp), whereas lanes; 1,3,5,7,9,11 in Figure 5A indicated six fragments (509 bp, 96 bp, 169 bp, 107 bp, 105 bp, 73 bp respectively) of Mbov_0730, and lanes; 2,4,6,8,10,12 indicated negative control. Lane “1” in Figure 5B indicated amplification of complete gene Mbov_0730 (867 bp).