Table 8. List of primers used for the confirmation of M. bovis HB0801, and M. bovis-150 strain and in overlap extension PCR for site-directed mutagenesis.

Primers	Sequence (5′→3′)	Remarks
uvrC-F	TAATTTAGAAGCTTTAAATGAGCGC	Confirmation of M. bovis specie
uvrC-R	CATATCTAGGTCAATTAAGGCTTTG
Mbov_0732-F	AGCGACCAAAATACTAGAC	Confirmation of vaccine strain i.e. M. bovis-150
Mbov_0732-R	TCGTTGCCACTGTATTCA
P730 1	ACGGAATTCATGCCTAATGATGGTTCA	EcoR I site
P730 2	ACAATTTTTTTGCCATTTTTTATACTGATCCAGTTTTTA	UGA to UGG
P730 3	ATAAAAACTGGATCAGTATAAAAAATGGCAAAAAAATTGT
P730 4	GAAGAACAGTGAGCTTAATTCCTGTCCAATC
P730 5	CACAAAGATTGGACAGGAATTAAGCTCACT
P730 6	CAGCATACTTAATATTTGATGTATCCCATTCATTAAGGTT
P730 7	AACCTTAATGAATGGGATACATCAAATATTAAGTATGCTG
P730 8	ACGCTACCTCTAATTTTCCAATTTTTTAAAGACTGATCTA
P730 9	TTTAAAAAATTGGAAAATTAGAGGTAGCGTTAATACCAAG
P730 10	GTTTTCCAAGCGGTAGCCATATCCTT
P730 11	AAAGGATATGGCTACCGCTTGGAAAAC
P730 12	GCCAAGCTTTTATGCTTTTTTATAGTTATATAGCATATTT	Hind III site