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. 2018 Jun 19;9(47):28731–28744. doi: 10.18632/oncotarget.25618

Figure 5. Mcl-1 downregulation by RO3280 increase TRAIL apoptotic activity in NSCLC cells.

Figure 5

Cells were cultured with 50 nM RO3280 and 20 ng/ml rhTRAIL (A) or 5, 50, 500 nM RO3280 for 24 h (B) The cell lysates were analyzed by western blot with indicated antibodies and immunoblot bands values are shown in histograms (n = 3, ± SEM) (A, B). Cells were transfected with an Mcl-1 encoding plasmid or a control plasmid 24 hrs prior to treatment (C). The cells were further treated with RO3280 (50 nM) and rhTRAIL (20 ng/ml). After 24 hrs, cell lysates were analyzed by western blot. PARP cleavage normalized by β-Actin (C).