Figure 6. The influence of RO3280/rhTRAIL combined treatment on the signaling pathway in NSCLC cells.

H1975, PC9, and A549 cells were treated with a combination of RO3280 (50 nM) and rhTRAIL (20 ng/ml) for 24 hrs and cell lysates were analyzed by western blot with indicated antibodies (A). Quantification of immunoblot analysis blots are shown in histogram (n = 3, ± SEM) (A). Cells were transfected with STAT3 plasmid or a control plasmid 24 hrs prior to drugs treatment and were further treated with RO3280 (50 nM) and rhTRAIL (20 ng/ml) (B). After 24 hrs, cell lysates were analyzed by western blot with indicated antibodies (B). PARP cleavage normalized by β-Actin were quantified by Image studio. Cell lysates for each cell line were run in the same gels (B).