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Polarized HBE cell monolayers were incubated with DrBoNT-488 and Hn33-PacBlue for 1.5 h. Cells were fixed and then imaged. (a) Hn33-PacBlue (red); (b) DrBoNT-488 (green), and (c) Hn33-PacBlue and DrBoNT-488 merged. Arrows point to the punctations showing colocalization. (Manders coefficient = 0.6) Red bar = 2 μM.
