Fig 6. Western blot revealed GFP expression 5 days post-transfection.

Sf9 cells were transfected with the different bacmid constructs as indicated at the top of the rows. GFP protein was not detectable, as expected, for the WT AAV2 baculovirus. Rep78eGFP and Rep52eGFP were detected at the expected sizes, respectively. GFP driven by the p5 promoter appeared at 27 kDa, and with the Rep constructs a 32 kDa band was clearly detected.