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(A)aAPC based on the K562 cell line encoding a non-signaling EGFRvIII mutant. (B)aAPC based on the K562 cell line encoding a non-signaling EGFRvIII mutant in addition to CD32, CD80, and CD137L. (C)G1-EGFRvIII CAR (D)G3-EGFRvIII CAR (E)Overall transduction and stimulation protocol for (F)three week and (G)six week (under selection) of CAR T-cells.
