Figure 2. uORF length (L) influences how the flux of scanning ribosomes changes downstream (rout) in response to lowered ribosome load (rin) due to stress.
Other parameters in the simulations: tε>σ=0; tσ>ε=0.8; mσ=mε=0.3. (A, B) Relative (rout[rin]/rout[rin=0.1]) (A) and absolute (B) changes in the density of ribosomes downstream of a uORF for a range of rin from 0.1 to 0 for mRNAs containing uORFs of different lengths (L). (C) Dependence of the relative (rout) maximum on the uORF length. (D) The decrease of maximum rout as a result of increasing uORF length (maximum rout for each L is normalized by rout for L= 2). Source data for panel (A) are provided in Figure 2—source data 1 and for panel (B) in Figure 2—source data 2.
Figure 2—source data 1. Source data for Figure 2A.
elife-32563-fig2-data1.txt (6.4KB, txt)
DOI: 10.7554/eLife.32563.004
Figure 2—source data 2. Source data for Figure 2B.
elife-32563-fig2-data2.txt (6.4KB, txt)
DOI: 10.7554/eLife.32563.005