Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila
melanogaster) |
S-18a-13b-16b.1 | PMID: 24935095 | starter stock used for EMS mutagenesis; genotype: y[*] w[*]; P{RedH-Pelican.org-1-HN18-dsRed, w[+mC]}18a, P{pGD130.tinC*-GFP, y[+]}13b, P{RedH-Pelican.HLH54Fb- dsRed, w[+mC]}16b |
|
| Genetic reagent (D. melanogaster) | S-18a-13b-16c.1 | PMID: 24935095 | starter stock used for EMS mutagenesis; genotype: y[*] w[*]; P{RedH-Pelican.org-1-HN18-dsRed, w[+mC]}18a, P{pGD130.tinC*-GFP, y[+]}13b, P{RedH-Pelican.HLH54Fb- dsRed, w[+mC]}16 c |
|
| Genetic reagent (D. melanogaster) | aop[1] | Bloomington Drosophila Stock Center |
BDSC:3101 | |
| Genetic reagent (D. melanogaster) | bib[S1538] | this paper | mutation in S-18a-13b-16c.1 background |
|
| Genetic reagent (D. melanogaster) | Df(2R)edl-S0520 | this paper | mutation in S-18a-13b-16b.1 background |
|
| Genetic reagent (D. melanogaster) | edl[k06602] | Bloomington Drosophila Stock Center |
BDSC:10633; FBal0057093 |
|
| Genetic reagent (D. melanogaster) | Df(2R)edl-L19 | Y. Hiromi, PMID: 12874129 | FBab0037748 | |
| Genetic reagent (D. melanogaster) | P{edl.AF1}BS12; P{edl[+]} | this paper | derived from injection with pCaSpeR4-X18C12-edl_rescue; line # BS12 carries P{edl.AF1} on chromosome 3 |
|
| Genetic reagent (D. melanogaster) | Egfr[f2] | Bloomington Drosophila Stock Center |
BDSC:2768 | |
| Genetic reagent (D. melanogaster) | Egfr[S0167] | this paper | mutation in S-18a-13b-16b.1 background |
|
| Genetic reagent (D. melanogaster) | Egfr[S2145] | this paper | mutation in S-18a-13b-16c.1 background |
|
| Genetic reagent (D. melanogaster) | Egfr[S2307] | this paper | mutation in S-18a-13b-16c.1 background |
|
| Genetic reagent (D. melanogaster) | Egfr[S2561] | this paper | mutation in S-18a-13b-16c.1 background |
|
| Genetic reagent (D. melanogaster) | htl[YY262] | PMID: 8957001 | ||
| Genetic reagent (D. melanogaster) | mam[S0669] | this paper | mutation in S-18a-13b-16b.1 background |
|
| Genetic reagent (D. melanogaster) | mam[S4648] | this paper | mutation in S-18a-13b-16c.1 background |
|
| Genetic reagent (D. melanogaster) | mid[1] | Bloomington Drosophila Stock Center |
BDSC:3086 | |
| Genetic reagent (D. melanogaster) | mid[S0021] | this paper | mutation in S-18a-13b-16b.1 background |
|
| Genetic reagent (D. melanogaster) | midE19-GFP | M. Frasch; PMID: 23326246 | ||
| Genetic reagent (D. melanogaster) | mid180-GFP | this paper | insertion in attP2 | |
| Genetic reagent (D. melanogaster) | mid180-mETS-GFP | this paper | insertion in attP2 | |
| Genetic reagent (D. melanogaster) | pnt[MI03880] | Bloomington Drosophila Stock Center |
BDSC:37615 | |
| Genetic reagent (D. melanogaster) | pnt[Δ88] | Bloomington Drosophila Stock Center |
BDSC:861 | |
| Genetic reagent (D. melanogaster) | pyr[18] | PMID: 19515694 | ||
| Genetic reagent (D. melanogaster) | pyr[S3547] | PMID: 22609944 | ||
| Genetic reagent (D. melanogaster) | rho[7M43] | Bloomington Drosophila Stock Center |
BDSC:1471 | |
| Genetic reagent (D. melanogaster) | rho[L68] | Bloomington Drosophila Stock Center |
BDSC:9095 | |
| Genetic reagent (D. melanogaster) | S[S4550] | this paper | mutation in S-18a-13b-16c.1 background |
|
| Genetic reagent (D. melanogaster) | S[B0453] | F. Schnorrer; PMID: 18327265 |
||
| Genetic reagent (D. melanogaster) | spi[S3384] | this paper | mutation in S-18a-13b-16c.1 background |
|
| Genetic reagent (D. melanogaster) | spi[1] | Bloomington Drosophila Stock Center |
BDSC:1859; FBal0016005 |
|
| Genetic reagent (D. melanogaster) | svp[AE127]-lacZ | Y. Hiromi, PMID: 11404079 | ||
| Genetic reagent (D. melanogaster) | ths[759] | PMID: 19515694 | ||
| Genetic reagent (D. melanogaster) | ‘tin-ABD;tin[EC40]’ | PMID: 16987868 | ||
| Genetic reagent (D. melanogaster) | UAS-aop.ACT-IIa | Bloomington Drosophila Stock Center |
BDSC:5789 | |
| Genetic reagent (D. melanogaster) | UAS-edl-X | Y. Hiromi, PMID: 12874129 | ||
| Genetic reagent (D. melanogaster) | ‘UAS-Egfr[DN].B-29-77-1; UAS-EgfrDN.B-29-8-1’; 2x EGFR[DN] |
Bloomington Drosophila Stock Center |
BDSC:5364 | |
| Genetic reagent (D. melanogaster) | UAS-mid-B2 | PMID: 15922573 | ||
| Genetic reagent (D. melanogaster) | UAS-pntP1-3 | Bloomington Drosophila Stock Center |
BDSC:869 | |
| Genetic reagent (D. melanogaster) | UAS-pntP2[VP16]−2 | C. Klämbt; PMID: 11051548 |
||
| Genetic reagent (D. melanogaster) | UAS-p35 | Bloomington Drosophila Stock Center |
BDSC:5073 | |
| Genetic reagent (D. melanogaster) | UAS-rho[EP3704] | Bloomington Drosophila Stock Center |
BDSC:17276 | |
| Genetic reagent (D. melanogaster) | UAS-rho(ve.dC) | Bloomington Drosophila Stock Center |
BDSC:8858 | |
| Genetic reagent (D. melanogaster) | UAS-svp | M. Hoch | ||
| Genetic reagent (D. melanogaster) | how[24B]-GAL4; 24B | Bloomington Drosophila Stock Center |
BDSC:1767 | |
| Genetic reagent (D. melanogaster) | tinCΔ4-GAL4 | M. Frasch; PMID: 11404079 |
||
| Genetic reagent (D. melanogaster) |
tinD-GAL4 | J. Weiss; PMID: 16221729 |
||
| Genetic reagent (D. melanogaster) |
2xPE-twi-GAL4 | Bloomington Drosophila Stock Center |
BDSC:2517 | |
| Genetic reagent (D. melanogaster) |
Df(2L)Exel6006 | Bloomington Drosophila Stock Center |
BDSC:8000 | |
| Genetic reagent (D. melanogaster) |
Df(2R)BSC25 | Bloomington Drosophila Stock Center |
BDSC:6865 | |
| Genetic reagent (D. melanogaster) |
Df(2R)Exel7157 | Bloomington Drosophila Stock Center |
BDSC:7894 | |
| Genetic reagent (D. melanogaster) |
Df(3R)Exel9012 | Bloomington Drosophila Stock Center |
BDSC:7990 | |
| Genetic reagent (D. melanogaster) |
lbe-GFP | Bloomington Drosophila Stock Center |
BDSC:55822 | |
| Genetic reagent (D. melanogaster) |
pnt-GFP | Bloomington Drosophila Stock Center |
BDSC:42680 | |
| Recombinant DNA reagent |
pCaSpeR4-X18C12- edl_rescue (plasmid) |
Y. Hiromi, P MID: 12874129 |
P transformation plasmid for generation of P{edl.AF1} |
|
| Antibody | anti-Doc2+3 (guinea pig polyclonal) |
PMID: 12783790 | (1:2000, TSA) | |
| Antibody | anti-Doc3+2 (guinea pig polyclonal) |
PMID: 12783790 | (1:1000) | |
| Antibody | anti-H15 (rabbit polyclonal) |
J. Skeath; PMID: 19013145 |
(1:2000) | |
| Antibody | anti-H15 (guinea pig polyclonal) |
J. Skeath; PMID: 19013145 |
(1:2000) | |
| Antibody | anti-Mid (rabbit polyclonal) |
J. Skeath; PMID: 19013145 |
(1:250, TSA or 1:1000) | |
| Antibody | anti-PntP1 (rabbit polyclonal) |
J. Skeath; PMID: 12756183 |
(1:250) | |
| Antibody | anti-Mef2 (rabbit polyclonal) |
H.T. Nguyen | (1:1500) | |
| Antibody | anti-Odd (rat polyclonal) |
PMID: 9683745 | (1:600, TSA) | |
| Antibody | anti-Eve (rabbit polyclonal) |
PMID: 2884106 | (1:3000) | |
| Antibody | anti-Tin (rabbit polyclonal) |
PMID: 9362473 | (1:750) | |
| Antibody | anti-Zfh1 (rabbit polyclonal) |
R. Lehmann; PMID: 9435286 |
(1:2000) | |
| Antibody | anti-dpMAPK (mouse monoclonal) |
Sigma | (1:500, TSA) | |
| Antibody | anti-Seven-up 5B11 (mouse monoclonal) |
Developmental Studies Hybridoma Bank |
(1:20, TSA) | |
| Antibody | anti-Wg 4D4 (mouse monoclonal) |
Developmental Studies Hybridoma Bank |
(1:30, TSA) | |
| Antibody | anti-β-galactosidase 40- 1a (mouse monoclonal) |
Developmental Studies Hybridoma Bank |
(1:50, TSA or 1:20) | |
| Antibody | anti-β-galactosidase (rabbit polyclonal) |
Cappel | (1:1500) | |
| Antibody | anti-GFP (rabbit polyclonal) |
Molecular Probes | Molecular Probes:A6455 |
(1:2000) |
| Antibody | anti-GFP (rabbit polyclonal) |
Rockland | Biomol:600-401-215 | (1:1000) |
| Antibody | anti-GFP 3E6 (mouse monoclonal) |
Life Technologies | Life Technologies:A11120 | (1:100, TSA) |
| Antibody | anti-cleaved-Caspase-3 Asp175 (rabbit polyclonal) |
Cell Signaling Technology | Cell Signaling Technology:#9661 |
(1:100, TSA) |
| Antibody | sheep anti-Digoxigenin (sheep polyclonal) |
Roche | Roche:11333089001 | (1:1000, TSA) |
| Commercial assay or kit |
VectaStain Elite ABC-HRP kit |
Vector Laboratories | Linaris:PK-6100 | |
| Commercial assay or kit |
tyramide signal amplification (TSA) reagent Cy3 |
PerkinElmer | PerkinElmer: SAT704A001EA |
|
| Commercial assay or kit |
tyramide signal amplification (TSA) reagent Fluorescein |
PerkinElmer | PerkinElmer: SAT701001EA |
|
| Commercial assay or kit |
TUNEL apoptosis detection kit (Apoptag) |
Millipore | Millipore:S7100 |