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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Hepatology. 2018 May 10;68(1):258–273. doi: 10.1002/hep.29787

Figure 8. GR antagonist (RU-486) abrogates rhRLX-induced hepatoprotection in IR-stressed mouse OLT.

Figure 8

Mouse livers subjected to 18h of cold storage were transplanted to syngeneic recipients treated with rhRLX or lactate ringer (control) at the time of reperfusion. Separate donor livers were pre-incubated with UW containing GR antagonist (RU-486, 500nM) for 18h during cold storage. (A) Representative hematoxylin and eosin staining (upper left, original magnification, x100) and Suzuki’s histological IRI grading (upper right, n=3–5/group). Representative TUNEL staining (lower left, original magnification, x400) and quantification of TUNEL-positive cells/HPF (n=3–4/group) (B) Serum ALT and AST levels (IU/L, n=3–5/group). (C) Western blot-assisted detection and relative intensity ratio of Bcl-2, Bcl-xL and cleaved caspase 3. β-actin expression served as an internal control and used for normalization (n=3/group). Data shown as mean±SD (Student t-test).