(A) 2D visualization of (10X Genomics) data from 899 HuH1 cells, 2088 HuH7 cells and 860 freshly isolated primary HCC cells by t-SNE. (B) t-SNE plots showing expression and distribution of hepatic CSC related genes. (C) HCC associated genes. (D) stemness-associated genes or immune cell genes. For panels B–D, each cell was colored based on their normalized expression of indicated genes, i.e., a gradient of gray, yellow, and red indicating low to high expression.