Table 1.
Primers and PCR conditions used for the identification and screening of virulence and biogenic amine genes
| Target gene | Primer (5′–3′) | AT (°C) | Size (bp) | References |
|---|---|---|---|---|
| Genetic markers | ||||
| 16S rRNA | 27F: AGAGTTTGATCCTGGCTCAG | 50 | 1500 | Gong et al. (2010) |
| 1492R: ACGGCTACCTTGTTACGACTT | ||||
| recA | planF: CCG TTTATGCGGAACACCTA pREV: CGGGATTACCA AAC ATCAC |
56 | 322 | Torriani et al. (2001) |
| dnaK | Lpdnak-500F3: CCGTTCTTRTCRATRTCRAA | 58 | 1100 | Huang et al. (2010) |
| Lpdnak-1710R5: GAAAYY CAAGTYGGHGAAGT | ||||
| Virulence genes | ||||
| ace | ACE-F: GAATTGAGCAAAAGTTCAATCG | 56 | 1008 | Martín-Platero et al. (2009) |
| ACE-R: GTCTGTCTTTTCACTTGTTTC | ||||
| asa1 | ASA11: GCACGCTATTACGAACTATGA | 56 | 375 | Vankerckhoven et al. (2008) |
| ASA12: TAAGAAAGAACATCACCACGA | ||||
| cylL S | CylLS1: GAAGCACAGTGCTAAATAAGG | 55 | 580 | Semedo et al. (2003) |
| CylLS2: GTATAAGAGGGCTAGTTTCAC | ||||
| efaA fs | TE5: GACAGACCCTCACGAATA | 54 | 705 | Eaton and Gasson.(2001) |
| TE6: AGTTCATCATGCTGTAGTA | ||||
| gelE | GEL11: TATGACAATGCTTTTTGGGAT | 45 | 213 | Vankerckhoven et al. (2008) |
| GEL12: AGATGCACCCGAAATAATATA | ||||
| hyl | HYLn1: ACAGAAGAGCTGCAGGAAATG | 56 | 576 | Vankerckhoven et al. (2008) |
| HYLn2:GACTGACGTCCAAGTTTCCAA | ||||
| Biogenic amine genes | ||||
| hdc | HIS1-F: GGNATNGTNWSNTAYGAYMGNGCNGA | 46 | 372 | de las Rivas et al. (2006) |
| HIS1-R: ATNGCDATNGCNSWCCANACNCCRTA | ||||
| tdc | TDC-F: TGGYTNGTNCCNCARACNAARCAYTA | 55 | 825 | de las Rivas et al. (2006) |
| TDC-R: ACRTARTCNACCATRTTRAARTCNGG | ||||
| odc | PUT1-F:GTNTTYAAYGCNGAYAARCANTAYTTYGT | 54 | 1446 | de las Rivas et al. (2006) |
| PUT1-R: ATNGARTTNAGTTCRCAYTTYTCNGG | ||||
| ldc | CAD2-R: CAYRTNCCNGGNCAYAA | 52 | 1185 | de las Rivas et al. (2006) |
| CAD2-F: GGDATNCCNGGNGGRTA | ||||
AT annealing temperature, bp base pair