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. 2018 Jul 5;8:10216. doi: 10.1038/s41598-018-26905-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alaskan blueberry polyphenols reduced expression of α-synuclein in the OW13 strain of C. elegans through a Sirtuin mediated pathway. (A) Graphical representation of fluorescence intensity of the day 7 OW13 animals fed on different genetic RNA interference treatments (L4440, sir-2.1 and daf-16) and Alaskan bog blueberry (0 and 400 μg/ml) from larval L4 stage. Empty vector L4440 was considered as a control. The data represent the mean ± SEM (n = 20–25 animals per group) with significant differences between the control and treatments at **p < 0.001 and ***p < 0.0001. Each experiment was repeated three times. (B) Representative confocal images of the α-synuclein/YFP expression in the head region of day 7 OW13 animals, magnification 40X and scale bar 50 μm.