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. 2018 Jul 5;8:10216. doi: 10.1038/s41598-018-26905-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduction of sir-2.1 expression reverses the motility improving effects of Alaskan bog blueberry polyphenols in aged (Day 12) OW13 strain of C. elegans. Percent per motility class of middle age ((day 5 adults; (A) and old age (day 12 adults; (B)) animals treated with different genetic RNA interference treatments (L4440, sir-2.1 and daf-16) and Alaskan bog blueberry extract (0 and 400 μg/ml) from larval L4 stage. Empty vector L4440 was used as control. Class A animals (dark gray) moved normally and spontaneously, class B animals (light gray) moved abnormally and may have required prodding, and class C animals (black) were unable to move or moved just head or tail in response to touch (n = 20 animals per group). Each experiment was repeated at least three times.