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. 2018 Jul 5;8:10216. doi: 10.1038/s41598-018-26905-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alaskan bog blueberry increased lipid content by a marginal increase in reactive oxygen species in OW13 strain of C. elegans. (A) Graphical representation for fluorescence intensity for Nile red staining of day 7 wild-type N2 (control) and OW13 animals fed Alaskan bog blueberry extract (0 and 400 μg/ml) from larval L3 stage. The data represent the mean ± SEM (n = 20–25 animals per group) with significant differences between the control and treatments at ***p < 0.001. Each experiment was repeated at least three times. (B) Representative confocal images of the intestine of day 7 wild-type N2 (control) and OW13 animals fed Alaskan bog blueberry extract (0 and 400 μg/ml), magnification 20X and scale bar 50 μm. (C) The change in endogenous ROS was measured by DCF-DA assay at day 7 of adulthood after treatment with crude extract of blueberries (0 and 400 μg/ml). Bars represent mean ± SEM of each replicate, with three technical replicates for the DCF-DA assay. #p = 0.07.